Streptococcus agalactiae|BNCC185941 |BNCC

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Streptococcus agalactiae

Culture medium	Brain heart extract medium (BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;24-48h; Aerobic;
Growth characteristics	G + ovalium, negative for arginine dihydrolase, negative for catalase and urease, grows in 10% bile, fermenting glucose, sucrose, trehalose, non-fermenting lactose, melibiose, arabinose, mannitol, sorbitol. Facultative anaerobic, optimum pH7.4 ~ 7.6. Antigenic properties: Lansfield Group A, non-hemolytic.
Storage conditions	2-8 ℃
morphology	Size: 0.5-1mm shape: round edge: neat transparency: opaque color: off-white bulge: middle bulge surface: smooth and bright texture: wet and easy to pick
application	Taxonomic studies, quality control strains.
Sharing mode	Public welfare sharing

Streptococcus agalactiae

No. 185941

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level: 2 , handle in safety cabinet

Storage conditions: 2~8 ℃

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, BHI: 4.0g of bovine brain extract powder, 4.0g of bovine heart extract powder, 5.0g of peptone, 16.0g of casin peptone, 5.0g of NaCl, 2.0g of glucose, 2.5g of disodium hydrogen phosphate, pH7.4 ± 0.2. 121 ℃,15min.

Recovery steps:

(1)Prepare a flask of BHI liquid media or two BHI agar plates.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ ; or directly dispense 200ul of the liquid suspension into a BHI agar plate evenly, then put the plates in incubator at 37℃ for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 24h BHI bacterial liquid is turbid, BHI strain layer is obvious
colony morphology: (above)	Size: Smaller Color: White Shape: Round Edge: Neat Edge Surface: Smooth and Bright Transparency: Opaque Texture: Slightly Dry
Conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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