Aspergillus brasiliensis|BNCC185739 |BNCC

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Aspergillus brasiliensis

Culture medium	CDA: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1.0L of distilled water and 6.2±0.2 of pH. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on Czapek's agar medium, the initial hyphae are white, dense and vigorous, and the later black spores are covered with plates.
application	Anti-mildew and antibacterial. GBT 1741 "Detection of Mold Resistance to Mold in Paint Film"
Sharing mode	Public welfare sharing

Aspergillus brasiliensis

Storage conditions: 2~8 ℃

No. 185739

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28℃, aerobic, Czapek's agar, 5-7 days, Czapek's agar: sucrose 30.0 g, NaNO3 3.0 g, MgSO4 7H2O 0.5 g, KCl 0.5 g, FeSO4 4H2O 0.01 g, K2HPO4 1.0 g, agar 15.0 g, distilled water 1.0 L, pH 6.0-6.5, 121℃, 15min. 121℃, 15min.

Recovery steps:

①Prepare 1-2 of above mentioned plates;

②Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation 5-7days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 6days strain layer become obvious
colony morphology: (above)	small filamentous fungus, in Czapek ' The colony on sagar medium is obvious, the initial hyphae are white, dense and vigorous, and the later black spores are covered with plates
Conclusion:	good viability, no abnormal colony morphology, qualified

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