Clostridium butyricum|BNCC185375 |BNCC

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Clostridium butyricum

Culture medium	Liquid thioglycolate medium (FT): 15.0g of caseptone (trypsin hydrolysis), 5.0g of yeast extract, 5.0g of glucose, 0.5g of sodium thioglycolate, 0.5g of L-cystine, 2.5g of sodium chloride, 0.001g of azure, 0.75g of agar, 1.0L of distilled water and 7.1±0.2 of pH value. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;24-48h; Anaerobic;
Storage conditions	2-8 ℃
morphology	Clostridium butyricum, thioglycolate liquid culture medium test tube bacteria liquid turbid, microscopic examination for gram-positive bacilli.
application	Research; Production. Produce isopropanol.
Sharing mode	Public welfare sharing

Clostridium butyricum

Storage conditions : 2~8 ℃

No. : 185375

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 37 ℃, anaerobic, thioglycolate liquid medium, 24-48h. Thioglycolate liquid medium: caseptone (trypsin hydrolysis) 15.0g, yeast extract 5.0g, glucose 5.0g, sodium thioglycolate 0.5g,L-cystine 0.5g, sodium chloride 2.5g, azure 0.001g, agar 0.75g, distilled water 1.0L,pH 7.1 ± 0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

① Prepare 1 φ18mm test tube liquid culture medium, about 10mL (placed in anaerobic environment for 24 hours in advance);

② disinfect the surface of the ampoule tube, open it in the safety cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning place to break it, and then knock it open with tweezers;

③ suck about 0.5mL of liquid culture medium into lyophilized tube, fully dissolve, suck back into test tube liquid culture medium, and mix well;

④ place the liquid test tube in the above-specified Culture conditions Under culture, the bacterial liquid can be used if it is turbid or there is a large amount of bacterial growth at the bottom. Microscopic examination of bacteria liquid

Recovery record: activation is carried out according to activation requirements, and the recording results are as follows:

Item	test results
viability:	good viability, in 24 hours liquid medium become turbid
resurrection effect and microscopic examination: (above)	Clostridium butyricum, thioglycolate liquid culture medium test tube bacteria liquid turbid, microscopic examination for gram-positive bacilli
Conclusion	good viability, no abnormal colony morphology, qualified

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