Cordyceps militaris|BNCC185316 |BNCC

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Cordyceps militaris

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	25 ℃;5-7 days; Aerobic;
Growth characteristics	15 days long full slope, hyphae white, thick, rich, hyphae fluffy. The liquid submerged fermentation temperature is 23-25 ℃, the pH value is 5.5-7, and the fermentation period is 3-5 days.
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, the colony is obvious on the comprehensive PDA medium, white, the hyphae are villous, dense and vigorous, and the back of the medium is light yellow.
application	Research; Production. Health care, medicinal, suitable for liquid fermentation to produce mycelium.
Sharing mode	Public welfare sharing

Cordyceps militaris

Storage conditions : 2~8 ℃

No. : 185316

Product format :agar slant in 14mm test tube

Validity : growing culture, in 30 days

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 25°C, aerobic, integrated PDA, 4 days. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

① Prepare 1-2 integrated PDA tablets;

②After disinfection of the inclined surface of the test tube, open it in the safety cabinet;

③ cut into pieces with sterile inoculation hoe and inoculation shovel (see attached page),0.5×0.5cm2 small square block;

④ Place the small square block flat on the center of the plate and on the agar surface;

⑤ The plate is being cultured under the above culture conditions, and the strains can be used when they grow out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 4days strain layer become obvious
colony morphology:	small filamentous fungi, the colony is obvious on the comprehensive PDA medium, white, hyphae are villous, dense and vigorous, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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