Bipolaris sorokiniana|BNCC185286 |BNCC

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Bipolaris sorokiniana

Culture medium	Potato Glucose Agar (PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on PDA medium, gray-brown colonies, dense and vigorous, spreading round to the edge of the plate, and black on the back of the medium.
Separation substrate	Seeds
Sharing mode	Public welfare sharing

Bipolaris sorokiniana

Storage conditions : 2~8 ℃

No. : 185286

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours. if it is overdue, it will be regarded as good receiving goods. Dry powder shall not be retained after one use, and can be used after activating for 1~2 generations. please refer to the attached page for the use of inclined planes and flat plates. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions: 28 ℃, aerobic, PDA,5-7 days, PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (not included in liquid medium), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 5-7days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows

Item	test results
viability	good viability, in 5-7 days strain layer become obvious
colony morphology: (above)	small filamentous fungi, with obvious colonies on PDA medium, gray-brown colonies, dense and vigorous, spreading round to the edge of the plate, and black on the back of the medium.
Conclusion:	good viability, no abnormal colony morphology, qualified

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