BeNa Culture Collection
Culture medium | Streptomyces medium No. 2 (ISP-2): yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2 0.2. Sterilization at 121 ℃ for 15min. |
Subculture procedure | (1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions. |
Growth conditions | 28 ℃,5-7 days, aerobic |
Storage conditions | 2-8 ℃ |
morphology | Colony diameter is 2-4mm, irregular shape, irregular edge, opaque, yellow on the front, yellow on the back, convex in the middle, rough surface, wrinkled surface, firm and hard texture, dry texture, G + (blue-purple), bacilli, purity: pure |
Separation substrate | Red salmon |
application | Type strain; |
Sharing mode | Public welfare sharing |