Aspergillus penicilloides|BNCC151891 |BNCC

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Aspergillus penicilloides

Culture medium	CDA: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1.0L of distilled water and 6.2±0.2 of pH. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungus, with obvious colonies on Cha's agar medium, gray-white, dense and vigorous velvety hyphae, and light yellow later Spores, spreading to the edge of the plate, the back of the medium is light yellow.
Separation substrate	Feed
Sharing mode	Public welfare sharing

Aspergillus penicilloides

Storage conditions : 2~8 ℃

No. : 151891

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, Cha's agar medium, 5-7 days. Cha's agar medium: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of sodium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1.0L of distilled water, pH 6.2 & plusmn;0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Place the plate under the above culture conditions, and the strain can be used when it grows out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows

Item	test results
viability:	good viability, in 5days strain layer become obvious
colony morphology:	Small filamentous fungi, with obvious colonies on Cha's agar medium, gray-white, dense and vigorous velvety hyphae, later producing light yellow spores, spreading and growing to the edge of the plate, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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