Aspergillus glaucus|BNCC148756 |BNCC

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Aspergillus glaucus

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, the colony is obvious on the comprehensive PDA medium, the initial hyphae are white, dense and low, and the later hyphae turn gray green, There are rings and gray-green spores.
Sharing mode	Public welfare sharing

Aspergillus glaucus

Storage conditions : 2~8 ℃

No. : 148756

Product format :agar slant in 14mm test tube

Validity : growing culture, in 30 days

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, comprehensive PDA,5-7 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 *7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

① Prepare 1-2 integrated PDA tablets;

②After disinfection of the inclined surface of the test tube, open it in the safety cabinet;

③ cut into pieces with sterile inoculation hoe and inoculation shovel (see attached page),0.5×0.5cm2 small square block;

④ Place the small square block flat on the center of the plate and on the agar surface;

⑤ The plate is being cultured under the above culture conditions, and the strains can be used when they grow out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 7days strain layer become obvious
colony morphology:	small filamentous fungi have obvious colonies on comprehensive PDA medium, with white hyphae at the initial stage, dense and low flat, and gray-green hyphae at the later stage, with ring lines and gray-green spores.
Conclusion:	good viability, no abnormal colony morphology, qualified

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