Mucor circinellolides|BNCC147484 |BNCC

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Mucor circinellolides

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Spread growth, filamentous, irregular edges, opaque, gray-white front, gray-white back, flat shape, rough surface, dry texture, purity: pure
Sharing mode	Public welfare sharing

Mucor circinellolides

No.: 147484

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, 5-7 days, comprehensive PDA agar medium: potato boiling juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

activation steps:

① Prepare the above 2 plates;

②Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then break it with tweezers;

③ Suck 0.5mL of sterile water into the freeze-drying tube and mix well;

④ Absorb 0.2mL of bacterial suspension into the plate, coat evenly, repeat twice to obtain two plates;

⑤ All the plates are cultured under the above culture conditions, and the strains can be used when they grow out.

recovery quality record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 5-7 days strain layer become obvious, colony is typical
colony morphology: (above)	filamentous fungi have obvious colonies on comprehensive PDA culture medium, hyphae are gray-white, dense/long, hyphae spread vigorously and produce gray-white spores.
conclusion:	good viability, no abnormal colony morphology, qualified

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