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Aspergillus parasitism-BNCC
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  • Aspergillus parasitism-BNCC

Aspergillus parasitism

  • Price： $ 172
  • number：BNCC144221
  • Form：
    Small filamentous fungi, with obvious colonies on the integrated PDA medium, white, dense and vigorous hyphae, spreading and growing to the edge of the plate, The yellow-green spores are produced in the later stage, and the back of the medium is light yellow.
Standard strain Quantitative strain DNA extraction
Package：
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
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Essential Information Certificate Related Products
Aspergillus parasitism
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Growth conditions 28 ℃;5-7 days; Aerobic;
Storage conditions 2-8 ℃
morphology Small filamentous fungi, with obvious colonies on the integrated PDA medium, white, dense and vigorous hyphae, spreading and growing to the edge of the plate, The yellow-green spores are produced in the later stage, and the back of the medium is light yellow.
Separation substrate Rice
Sharing mode Public welfare sharing

Aspergillus parasiticus

Storage conditions: 2~8 ℃

No. 144221

Product format： freeze dried, 200ul 

Validity period: 6 years

Biosafety  level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28°C, aerobic, integrated PDA, 5-7 days. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, trace amount of vitamin B1, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates; 

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps; 

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate; 

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

Item test results
viability good viability, in 6 days plate colony is obvious
colony morphology:

small filamentous fungi with obvious colonies on comprehensive PDA medium, white,

The hyphae are dense and vigorous, spreading to the edge of the plate, producing yellow-green spores in the later stage, and the back of the medium is light yellow
Conclusion: good viability, no abnormal colony morphology, qualified
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