Ganoderma tsugae|BNCC143310 |BNCC

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Ganoderma tsugae

Culture medium	Potato Glucose Agar (English Name: PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	30 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on integrated PDA medium, white hyphae, dense and vigorous, concentric vines to the edge of the plate Extended growth.
Sharing mode	Public welfare sharing

Ganoderma tsugae

No. 143310

Storage conditions: 2~8 ℃

Product format: agar slant in 14mm test tube

Validity period: 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:30 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH₂PO₄ 3g, MgSO₄.7H₂O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4) Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability,in 7 days plate colony is obvious
colony morphology:	small filamentous fungi with obvious colonies on comprehensive PDA medium, hyphae are white, dense and vigorous, spreading to concentric circles on the edge of the plate.
Conclusion:	good viability, no abnormal colony morphology, qualified

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