BeNa Culture Collection

Brevundimonas diminuta-BNCC
Brevundimonas diminuta-BNCC
Brevundimonas diminuta-BNCC
  • BNCC
  • Brevundimonas diminuta-BNCC
  • Brevundimonas diminuta-BNCC
  • Brevundimonas diminuta-BNCC

Brevundimonas diminuta

  • Price: $ 172
  • number:BNCC138603
  • Form:
    Size: small color: light yellow shape: round edge: neat dry and wet: wet and smooth: smooth transparency: opaque uplift: uplift, bacillus, G-(red), purity: pure
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Brevundimonas diminuta
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃;24-48h; Aerobic;
Storage conditions 2-8 ℃
morphology Size: small color: light yellow shape: round edge: neat dry and wet: wet and smooth: smooth transparency: opaque uplift: uplift, bacillus, G-(red), purity: pure
application Check the retention effect of filter membrane (0.22 μm) on bacteria
Sharing mode Public welfare sharing

Brevundimonas diminuta

Storage conditions : 2~8 ℃

No. : 138603

Product format : freeze dried,200ul

Validity period: Freeze-dried tube for 6 years

Biosafety  level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture:37°C, aerobic, nutrient gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

(1)Prepare a flask of NB liquid media or two NA agar plates.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker   at 37℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a NA agar plate evenly, then put the plates in incubator at 37℃ for 24-48 hours.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                                              

item test result
viability good viability, in 20h NB bacterial fluid become turbid, bacterial fluid scribing plate colonies are obviously typical
colony morphology:

size: small color: light yellow shape: round edge: neat

wet and dry: wet and smooth: smooth transparency: opaque uplift: uplift

Conclusion: good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

 

 

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