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Vibrio cholerae is the pathogen of human cholera. Cholera is one of the ancient and widespread severe infectious diseases. The epidemic peak is mostly in July-October. The world is still in the seventh pandemic. If severe and typical patients are not treated in time, they can cause death.
The latest Vibrio cholerae ompW gene plasmid DNA reference material developed by BNCC is suitable for PCR-related detection of Vibrio cholerae ompW gene, which provides a good guarantee for the traceability of its value and can be used for relevant analysis in fields such as inspection and quarantine, disease control, and food safety control. Method evaluation, instrument calibration and quality control of measurement results, etc.
This product uses digital PCR for absolute quantification, and the homology is as high as 99.54% under identity verification. A total of 1 tube containing 100μL, in freeze-dried form, packaged in cryopreservation tubes, the product is shipped with a full set of certificates, and the quality is guaranteed.
Product code
Product name
Specifiction
BNCC360420
BNCC363235
Helicobacter pylori DNA standard
Frozen vial;(2.5±0.7)×10^5copies/mL;5vial/box
BNCC363236
Frozen vial;(2.5±0.7)×10^6copies/mL;5vial/box
BNCC363237
Frozen vial;(2.5±0.7)×10^7copies/mL;5vial/box
BNCC363413
Freeze dried;(2.2±0.6)×10^5copies/mL;5vial/box
BNCC363414
Freeze dried;(2.8±0.7)×10^6copies/mL;5vial/box
BNCC363415
Freeze dried;(2.5±0.7)×10^7copies/mL;5vial/box
BNCC363417
Frozen vial;10^6~10^7copies/mL;5vial/box
BNCC363418
Frozen vial;10^5~10^6copies/mL;5vial/box
BNCC363419
Frozen vial;10^4~10^5copies/mL;5vial/box
BNCC363450
Escherichia coli DNA Standard
Frozen vial;(5.3±1.0)×10^5copies/mL;5vial/box
BNCC363451
Frozen vial;(5.3±1.0)×10^6copies/mL;5vial/box
BNCC363452
Frozen vial;(5.3±1.0)×10^7copies/mL;55vial/box
Instruction:
This standard product should be taken out from the low temperature environment before use, and equilibrated to room temperature; please centrifuge (12000rmp, 60s) before opening the cover; add 100μL enzyme-free sterile water to dissolve after opening the cover, shake evenly, and use it after instantaneous centrifugation.
Preparation:
This standard product is the complete gene sequence of Vibrio cholerae outer model protein ompW cloned into the plasmid vector pUC19, and the plasmid DNA standard product containing the target gene sequence is constructed; Escherichia coli DH5α is used for replication and production, and the target plasmid DNA is extracted and purified. Dilute aliquots to obtain plasmid DNA products.
Notice:
1 After receiving the goods, please keep them properly. In case of damage, please contact us within 24 hours.
2 Please operate in a biological safety cabinet in a sterile environment to avoid repeated freezing and thawing.
3 When using the standard, attention should be paid to biosafety protection, and it should be treated as potentially bioinfectious samples. The operation and treatment should comply with relevant regulations.
Various bacteria have their own unique enzyme systems, so their ability to decompose substrates is different, and their metabolites are also different.
The coliphage MS2 nucleic acid reference product developed by BNCC belongs to the heat-inactivated coliphage MS2, which is a virus particle that loses the growth and infection and pathogenicity of the phage through high temperature, but retains the complete genetic material. Mainly used for quality control substances in the molecular biology detection process of RNA viruses, positive controls for nucleic acid detection, etc.