Vibrio parahaemolyticus and Vibrio cholerae nucleic acid test kit certificate

1. Product information

Sample name: Vibrio parahaemolyticus and Vibrio cholerae nucleic acid detection kit (fluorescent PCR method)

Sample number:  361346

Sample batch: 220420

2. Product features

3. Instruction

1. Experimental preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, and place on ice for later use;

2. Sample processing: sample processing according to "SN/T 5125-2019 Inspection of Vibrio parahaemolyticus and Vibrio cholerae";

3. Reagent preparation: experimental reaction system (25 μL), 21 μL qPCR mixed reaction solution, 4 μL template;

4. Reaction conditions: 95°C 3min 1cycle; 94°C 10s, 50°C 30s 40cycle;

5. Fluorescence channel: FAM (Vibrio parahaemolyticus), CY5 (Vibrio cholerae);

4. Result judgment

1. The sample has a typical amplification curve, and the Ct value is less than or equal to 35;

2. The sample has no amplification curve, and the Ct value is ≥40 or not detected;

3. If the t value of the sample is >35 and <40, it needs to be re-measured. Retest results Ct value ≥ 40 is negative, Ct value < 40 is positive.

5.Preservation and transportation

1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;

2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;

3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;

Henan Engineering Research Center of Industrial Microbiology

Website: www.bncc .org .cn Tel: 400-6699-833