Water quality enzyme substrate method negative quality control sample|BNCC360395 |BNCC

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Water quality enzyme substrate method negative quality control sample

Culture medium	MMO-MUG medium: composition (g/L): ammonium sulfate 5.0; Manganese sulfate 0.0005; Zinc sulfate 0.0005; Magnesium sulfate 0.1; Sodium chloride 10.0; Calcium chloride 0.05; Sodium sulfite 0.04; Amphotericin B 0.001; ONPG 0.5;MUG 0.075;Solanium extract 0.5;HEPES 6.9;HEPES sodium salt; Take 100ml of water sample from a 100ml sterile dilution bottle and add 2.7±0.5g of this product, shake evenly to completely dissolve it, and put it into an incubator at 36±1 ℃ for 24 hours.
Subculture procedure	Before use, the quality control sample is placed at room temperature for 30min, and the sample needs to be dissolved in a total of 100mL of sterile water or sterile normal saline. Then open it in the biological safety cabinet, immediately add 3~4mL of sterile water, dissolve it and suck it out into a sterile bottle, repeatedly wash the inner wall of the penile bottle with the remaining sterile water, and transfer it to the above sterile bottle, which is 100mL standard sample stock solution.
Growth conditions	37 ℃; Culture time 24-48 hours; Anaerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	2
application	Reference standard: HJ 1001-2018 Enzyme Substrate Method for Determination of Total Coliforms, Fecal Coliforms and Escherichia coli in Water Quality; Negative Control for Determination of Total Coliforms, Fecal Coliforms and Escherichia coli in Water Quality (Enzyme Substrate Method).
Sharing mode	Public welfare sharing

Certificate of Negative Quality Control Sample for Water Quality Enzyme Substrate Method

1. Product information

Sample name: Negative quality control sample for water quality enzyme substrate method

Sample number: 360395

Sample batch: 211203

2. Product features

description	Appearance is white powder (lyophilized powder), containing protective agent, packaged in 7mL vial
traceability	ATCC 27853 Pseudomonas aeruginosa
concentration	300~3000 CFU/mL
Reference value	2400 CFU/mL
Uniformity and stability	Random sampling, the results are uniform; long-term stability test, the stability is qualified
specific use	Negative control for determination of total coliforms, fecal coliforms and Escherichia coli in water quality (enzyme substrate method)
applicable standard	《HJ 1001-2018 Water Quality-Determination of Total Coliform, Fecal Coliform and Escherichia Coli-Enzyme Substrate Method》
*Note: If you have any questions about the quality control samples, please contact our center (BNCC) for help before use

3. Instructions

The samples require a total of 100L sterile water or sterile saline to dissolve. Before use, place the sample at room temperature for 30 minutes, then open it in a biological safety cabinet, add 3-4mL of sterile water immediately, dissolve it, suck it out and put it into a sterile bottle, and repeatedly wash the inner wall of the vial with the remaining sterile water, and transfer all the samples to the vial. The above sterile bottle is the standard sample stock solution.

4. Notes

1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. Please operate in a sterile environment, in a biological safety cabinet, and use it all at once after opening;

3. This product contains pathogenic microorganisms. Please do not directly touch the samples in the bottle with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them.

5. Preservation and transportation

long-term storage:-20 ℃, valid for 1 year; Transportation conditions: ice bag transportation, the time should not exceed 7 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn Tel: 400-6699-833

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