Quantitative strain of Pseudomonas aeruginosa|BNCC359609 |BNCC

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Quantitative strain of Pseudomonas aeruginosa

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	1. According to the arrow sign of penicillin bottle cap, open the strain and dissolved liquid; 2. transfer the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve; 3. After fully dissolved, it is 50 ~ 100CFU/ml, which is absorbed in proportion according to the requirements; 4. Please use it up immediately after opening and do not store it.
Growth conditions	37 ℃;18-24h; Aerobic;
Growth characteristics	The strain was cultured at 37 ℃ for 3 days on nutrient gravy agar medium. The bacteria were rod-shaped, 0.61 μm × 1.22~2.13 μm, Gram-negative and did not produce spores.
Storage conditions	-20 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: Light yellow Protuberance: Middle raised surface: Bright and smooth Texture: Wet and easy to stir up , G-(red), bacilli, purity: pure
Separation substrate	Sick material
application	Research; Analytical testing. Quality control strains of chinese pharmacopoeia, 2015 edition. 2020 edition of Pharmacopoeia-"General Principles 1100 Biological Inspection Method" for test strains of bacteria in Pharmacopoeia, culture medium "sensitivity inspection" for test strains, "method applicability test" for test strains, "counting medium applicability test and test sample counting method applicability test" for test strains, "medium applicability test and control bacteria test method applicability test" for test strains, microbial counting "medium applicability test and method applicability test" test strain control bacteria test "medium applicability test and method applicability test" test strain "medium applicability test, method applicability test, bacteriostatic potency test" test strain
Sharing mode	Public welfare sharing

Pseudomonas aeruginosa

Strain number: BNCC 125486 / CMCC10104

Product format: quantitative bacteria tablets 50- 100CFU、bottle

Product batch：22011201

Date of inspection:2022.01.13

Valid until:2023.01.11

Number of viable bacteria:(75±25)CFU/bottle

Sampling ratio:≥2%

Report number: RS22125486-1

Biosafety level: 2, handle in ultra-clear table

Items	test results	description
stain microscopy	gram negative and positive, cell morphology observed under microscope	G + bacilli
colony morphology	observation of single colony morphology	light yellow, moist, raised, with neat edges
purity	non-selective medium culture, colony observation and microscopic examination	No bacteria
Reference value of live bacteria	Non-selective medium plate, viable count	63CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

Product list: (75±25)CFU/bottle ,1 bottle of 50-100CFU quantitative strain, 1 bottle of 1mL lysate, 1 strain certificate;

Validity period: unopened, below -20 ℃, 12 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After fully dissolved, it is (75±25)CFU/mL, absorbed in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. Improper storage conditions and use methods will affect the accuracy of the number of strains, so please operate in strict accordance with the above storage conditions and methods.

②Used germ-carrying consumables should be discarded after autoclaving.

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