Quantitative strain of Escherichia coli|BNCC353780 |BNCC

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Quantitative strain of Escherichia coli

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	(1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions	37 ℃, aerobic
Storage conditions	-20 ℃
morphology	Pale yellow, raised, moist, bright, G-bacilli
Separation substrate	Clinical isolates
application	Sensitive test; Antibacterial test; Food testing; Cosmetic testing; Culture control; Quality control strain; API verification bacteria, GB 4789.38-2012 Escherichia coli count positive control strain.
Sharing mode	Public welfare sharing

Escherichia coli

No. : 353780 / ATCC 25922

Product format: quantitative bacteria tablets

Number of live bacteria : 2~3×10 3 CFU/bottle

Product batch : 201025

Valid until : 2021.10.26

Date of inspection : 2020.10.27

Sampling ratio : ≥2%

Report number : RS20353780-1

Biosafety level : 1,handle in ultra-clean table or safety cabinet

item	inspection description	result
staining microscopy	Gram negative and positive, observe cell morphology under microscope	G-bacilli, single rod
colony morphology	observe single colony morphology	milky white, raised, bright
purity	non-selective medium culture, colony observation and microscopic examination	no bacteria
reference value of live bacteria	non-selective medium plate, live bacteria count	2630CFU/bottle
phenotype and genotype analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control inspection system, the product is qualified

Product list :2~3×10 3 CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;

Validity period : unopened, below -20 ℃, 12 months;

Handling procedure:

1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 It will be 2~3×10 3cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Notes:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization

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