Xanthomonas campestris|BNCC359498 |BNCC

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Xanthomonas campestris

Culture medium	R2A agar medium (R2A): tryptone 0.25g, acid hydrolyzed casein 0.5g, yeast extract 0.5g, soluble starch 0.5g, dipotassium bisulfate 0.3g, magnesium sulfate 0.1g, sodium pyruvate 0.3 agar 12 peptone 0.25 glucose 0.5g,pH 7.2±0.2. 121 ℃,15min
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;18-24h; Aerobic;
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: Yellow Protuberance: Middle Protuberance Surface: Bright and Smooth Texture: Wet and Viscous
Sharing mode	Public welfare sharing

Xanthomonas campestris

Storage conditions : 2~8 ℃

No. : 359498

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, R2A agar medium, 18-24h. R2A agar medium: tryptone 0.25g, acid hydrolyzed casein 0.5g, yeast powder 0.5g, soluble starch 0.5g, dipotassium bisulfate 0.3g, magnesium sulfate 0.1g, sodium pyruvate 0.3g, agar 12g peptone 0.25g glucose 0.5g,pH7.2±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 18-24 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 24 hours strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque color: yellow uplift: middle convex surface: bright and smooth texture: moist and viscous
Conclusion	good viability, no abnormal colony morphology, qualified

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