BeNa Culture Collection

Metarhizium indigoticum (Kobayasi & Shimizu) Kepler et al.-BNCC
Metarhizium indigoticum (Kobayasi & Shimizu) Kepler et al.-BNCC
Metarhizium indigoticum (Kobayasi & Shimizu) Kepler et al.-BNCC
  • BNCC
  • Metarhizium indigoticum (Kobayasi & Shimizu) Kepler et al.-BNCC
  • Metarhizium indigoticum (Kobayasi & Shimizu) Kepler et al.-BNCC
  • Metarhizium indigoticum (Kobayasi & Shimizu) Kepler et al.-BNCC

Metarhizium indigoticum (Kobayasi & Shimizu) Kepler et al.

  • Price: $ 229
  • number:BNCC354185
  • Form:
    Filamentous fungi, the colony is obvious on the comprehensive PDA medium, dense and low, the initial hyphae are white, the later turn gray, and the gray-green spores are produced.
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Metarhizium indigoticum (Kobayasi & Shimizu) Kepler et al.
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 28 ℃;3-5 days; Aerobic;
Storage conditions 2-8 ℃
morphology Filamentous fungi, the colony is obvious on the comprehensive PDA medium, dense and low, the initial hyphae are white, the later turn gray, and the gray-green spores are produced.
Sharing mode Public welfare sharing

Metarhizium indigoticum

Storage conditions: 2~8 ℃

No. 354185

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, integrated PDA,3-5 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare the above 2 plates; 
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; 
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

Item test results
viability: good viability, in 3days strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above) filamentous fungi have obvious colonies on comprehensive PDA medium, dense and low, white hyphae at the initial stage, gray at the later stage, and gray-green spores.
Conclusion: good viability, no abnormal colony morphology, qualified
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