BeNa Culture Collection

Trichoderma longibrachiatum-BNCC
Trichoderma longibrachiatum-BNCC
Trichoderma longibrachiatum-BNCC
  • BNCC
  • Trichoderma longibrachiatum-BNCC
  • Trichoderma longibrachiatum-BNCC
  • Trichoderma longibrachiatum-BNCC

Trichoderma longibrachiatum

  • Price: $ 86
  • number:BNCC336352
  • Form:
    Filamentous fungi, the colony is obvious on the comprehensive PDA medium, the hyphae are white, dense/low, the hyphae are vigorous and spread, and yellow spores are produced.
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Trichoderma longibrachiatum
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 28 ℃;5-7 days; Aerobic;
Storage conditions 2-8 ℃
Safety level 1
morphology Filamentous fungi, the colony is obvious on the comprehensive PDA medium, the hyphae are white, dense/low, the hyphae are vigorous and spread, and yellow spores are produced.
Model strain no
application Cellulase production
Sharing mode Public welfare sharing

Trichoderma longibrachiatum

Storage conditions : 2~8 ℃

No. : 336352

Product Format: freeze dried, 200ul

Validity period: 6 years

Biosafety  level : 1,handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth Conditions :28 ℃, aerobic, 5-7 days, comprehensive PDA agar medium: potato boiling juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. Sterilization at 121 ℃ for 15min.     

Recovery steps:

(1)Prepare 2 of above mentioned plates;

(2)Open the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 

(3)Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; 

(4)Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; 

(5)Put  plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                                               

item test results
viability good viability, in 5-7 days strain layer is obvious and  colony is typical
colony morphology: (above) filamentous fungi have obvious colonies on comprehensive PDA medium, white hyphae, dense/low-flat hyphae, vigorous spread and yellow spores.
conclusion good viability, no abnormal colony morphology, qualified & nbsp;& nbsp;
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