BeNa Culture Collection

Streptococcus thermophilus Orla-Jensen-BNCC
  • BNCC
  • Streptococcus thermophilus Orla-Jensen-BNCC

Streptococcus thermophilus Orla-Jensen

  • Price: $ 372
  • number:BNCC319544
  • Form:
    Size: 1-2mm Shape: round Edge: not neat Transparency: Opacity Color: off-white Protuberance: Middle bulge Surface: Smooth Texture: moist and easy to stir up
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Essential Information Certificate Related Products
Streptococcus thermophilus Orla-Jensen
Culture medium Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃;18-24h;5% CO2 + 95% air;
Storage conditions 2-8 ℃
morphology Size: 1-2mm Shape: round Edge: not neat Transparency: Opacity Color: off-white Protuberance: Middle bulge Surface: Smooth Texture: moist and easy to stir up
Separation substrate Pasteurized milk
application Produces aminopeptidase N
Quality control strain
Sharing mode Public welfare sharing

Streptococcus thermophilus

Storage conditions: 2~8 ℃

No. 319544

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety  level:  1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃,5% CO2, Columbia blood plate (ready-to-use type),18-24h.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates; 

(2) Open it in  the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps; 

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate; 

(4) Put the plates under the above culture conditions for cultivation for 18-24 hours.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                                                              

Item test results
viability good viability, in 24 hours strain layer become obvious,  colony is typical
colony morphology: (above)

Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque

Color: off-white uplift: middle raised surface: smooth texture: moist and easy to stir

Conclusion good viability, no abnormal colony morphology, qualified
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