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Bacteroides caccae Johnson et al.
Storage conditions: 2~8 ℃
No. 232292
Product format: freeze dried, 200ul
Validity : 6 years
Biosafety level: 2 , handle in safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions :37 ℃, strictly anaerobic, improved GAM agar: 15.0g of monthly peptone, 10.0g of tryptone, 3.0g of soybean peptone, 5.0g of yeast powder, 2.0g of beef powder, 1.2g of beef liver powder, 0.3g of soluble starch, 0.15g of L-cysteine, 3.0g of glucose and 2.5g of potassium dihydrogen phosphate, digested serum powder 13.5g, sodium thioglycolate 0.15g, sodium chloride 3.0g, agar 13.0g, distilled water 1L,pH 7.0±0.2, 25 ℃ additive: 0.02% vitamin K1 solution and hemin
Recovery steps: (1) Prepare 2 of above mentioned plates; (2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) Draw 0.5ml of sterile water(keep in an anaerobic environment for 24 hours) into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate; (4) Put the plates under the above culture conditions for cultivation for 5-7 days. Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
good viability, in 48h strain layer is obvious;
colony is typical on the streaked plate
Size: 1-2mm Shape: Round Edge: Neat Transparency: Translucent
color: light yellow uplift: middle convex surface: bright and smooth texture: moist and easy to stir