Corynebacterium glutamicum|BNCC192544 |BNCC

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Corynebacterium glutamicum

Culture medium	Glutamic acid medium (GAFM): glutamic acid medium: peptone 10.0g, beef paste 10.0g, sodium chloride 5.0g, glucose 1.0g, agar 20g, distilled water 1L,pH6.8-7.0,121 ℃,15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;24-48h; Aerobic
Storage conditions	2-8 ℃
application	Production of glutamic acid.
Sharing mode	Public welfare sharing

1.Description

1. Name: Corynebacterium glutamicum

2. BNCC No.:192544

3. Biosafety level:4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1, Glutamic acid medium: peptone 10.0g, beef paste 10.0g, sodium chloride 5.0g, glucose 1.0g, agar 20g, distilled water 1L,pH6.8-7.0,121 ℃,15min.

2. Atmosphere:aerobic

3. Temperature: 30 ℃

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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