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BNCC > Microbial strain > Corynebacterium glutamicum
Corynebacterium glutamicum-BNCC
  • BNCC
  • Corynebacterium glutamicum-BNCC

Corynebacterium glutamicum

  • Price： $ 143
  • number：BNCC188178
Standard strain Quantitative strain DNA extraction
Package：
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
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Essential Information Certificate Related Products
Corynebacterium glutamicum
Culture medium Glutamic acid fermentation medium (GAFM): glutamic acid fermentation medium: peptone 10.0g, beef paste 10.0g, sodium chloride 5.0g, agar 20g, distilled water 1L,pH7.2,121 ℃,15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃;18-24h; Aerobic
Storage conditions 2-8 ℃
application Tianjin MSG factory produces bacteria to produce glutamic acid.
Sharing mode Public welfare sharing

1. Description 

1. Name: Corynebacterium sp

2. BNCC No.: 188178

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions: 

1, 617 bacteria (glutamic acid fermentation bacteria) culture medium: peptone 10.0g, beef paste 10.0g, sodium chloride 5.0g, agar 20g, distilled water 1L,pH7.2,121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature:  37 ℃

4. Notes: 

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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