Eurotium cristatum|BNCC146563 |BNCC

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Eurotium cristatum

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Filamentous fungi, with obvious colonies on the comprehensive PDA medium, yellow hyphae, dense and low flat, vigorous spread and growth of hyphae, and gray production Yellow spores.
Sharing mode	Public welfare sharing

Eurotium cristatum

No.: 146563

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level:1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours. if it is overdue, it will be regarded as good receiving goods. Dry powder shall not be retained after one use, and can be used after 1~2 generations of activation. please refer to the attached page for details of inclined plane and bacterial solution. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions:25-28 ℃, aerobic, 5-7 days, chashel agar medium: 3g sodium nitrate, 1g dipotassium hydrogen phosphate, 0.5g magnesium sulfate, 0.5g potassium chloride, 0.01g ferrous sulfate, 30g sucrose, 15g agar (not included in liquid medium), 1.0L distilled water, pH 6.2 ±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, directly dispense 200ul of the liquid suspension into a agar plate evenly.

(4) Put the plates under the above culture conditions for cultivation.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability,in 3 days floc growth at the top of the culture solution, obvious plate bacteria layer .In 5 days strain layer become obvious, colony is typical on the streaked plate
colony morphology: (above)	filamentous fungi, with obvious colonies on Cha's agar medium, forming tiny communities, hyphae are white, dense, hyphae spread and grow, producing a large number of gray-yellow spores.
conclusion:	good viability, no abnormal colony morphology, qualified

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