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Saccharomyces cerevisiae
Storage conditions : 2~8 ℃
No. : 140110
Product format : freeze dried,200ul
Validity period: freeze-dried tube 24 months
Biosafety level : 1, handle in ultra-clear table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions :25-28 ℃, aerobic, YM medium. YM medium: yeast extract, 3.0g malt extract, 3.0g glucose, 10.0g protein block, 5.0g agar, 20.0g distilled water 1000 ml ,pH 6.2 +/- 0.2,121 ℃,15min.
Recovery steps:
(1)Prepare 1-2 pieces of YM plates;
(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, directly dispense 200ul of the liquid suspension into a YM agar plate evenly.
(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
size: medium color: milky white shape: round edge: neat edge
wet and dry: wet and smooth: smooth and bright transparency: opaque uplift: bulge