BeNa Culture Collection

Human myelodysplastic syndrome cell line-BNCC
  • Human myelodysplastic syndrome cell line-BNCC
  • Human myelodysplastic syndrome cell line-BNCC
【MUTZ-1】

Human myelodysplastic syndrome cell line

  • Price: $416
  • number:BNCC360069
  • Form:
    Lymphoblast-like, round, single cell, clean background, no pigment, no vacuoles
Basic package DNA extraction
Package:
  • Package A:STR report + 2 vials of frozen cell
  • Package B:STR report + 2 vials of frozen cell + 100ml of complete medium
Essential Information Certificate Related Products
Human myelodysplastic syndrome cell line
Culture medium RPMI-1640 complete medium: 90% RPMI-1640 + 10% FBS
Subculture procedure Recovery steps: ① Take out the frozen vial from liquid nitrogen or -80 ℃ refrigerator and put it into PE gloves, quickly submerse into a 37 ℃ water bath, shake the frozen vial to accelerate dissolution, and it is advisable to dissolve all within 1min; (2) Add the dissolved cell solution into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm for 3-5min, discard the supernatant, resuspend the cells with 1-2ml of complete media. ③The cell suspension was then added to the T25 flask containing 6-7mL of complete medium and placed in an incubator for culture. The culture flask is recommended to be cultured uprightly. cell subculture: ① centrifugation: collect cells, centrifuge at 1000rpm for 5min, discard the supernatant, add 1-2mL of culture solution and blow well, and dispense the cell suspension into a fresh T25 flask containing 8mL of culture medium according to a ratio of 1:2. (2) Half-volume medium exchange method: half-volume medium exchange method can be selected; Please gently suck half of the supernatant culture medium, resuspend and mix the remaining culture medium with cell precipitation, and divide the cell suspension into a fresh T25 flask containing 8mL culture medium according to a ratio of 1:2. (3) Pay attention to the change of pH of medium and cell density, renew the medium regularly (2-3 times a week), and repeat the subculture or cryopreservation when the cell density reaches more than 2 × 106 cells/ml.
Growth conditions 37 ℃;5% CO2 + 95% air;
Growth characteristics Suspension growth
Storage conditions Liquid nitrogen
Type Lymphoma
Safety level 1
morphology Lymphoblast-like, round, single cell, clean background, no pigment, no vacuoles
Sharing mode Public welfare sharing

Human myelodysplastic syndrome cell line MUTZ-1

BNCC number: 360069

Growth characteristics: suspension growth

Growth conditions: 37 ℃,5% CO2

Complete medium: 90%RPMI-1640+10%FBS

Cryopreservation conditions: 50%RPMI-1640+40%FBS+10%DMSO

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the cells are well. For the frozen cells,  it shall be stored in the refrigerator at -80 ℃ upon arrival. If they are not used for a long time, they shall be transferred to liquid nitrogen for storage overnight.  Recovered cells in T25 culture flask, upon receipt, put the culture flask in the incubator for 2-3h, and then carry out handling procedure. Under aseptic conditions, transfer the original flask culture solution to a centrifuge tube, centrifuge at 1000-1200rpm/min for 5-10 min, resuspend the cells, transfer the suspended cells to a new T25 flask, add complete culture medium to 10ml, and put it into an incubator for culture. Please strictly follow this instruction, otherwise no reissue service will be provided if cell inactivation is caused.

Recovery steps: 
(1)The frozen vial is taken out of liquid nitrogen or -80 ℃ refrigerator and put into PE gloves, quickly submersed into a 37 ℃ water bath, shaken the frozen vial to accelerate dissolution, and it is advisable to dissolve all within 1min; 
(2)Put the dissolved cell solution into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm for 5min, discard the supernatant,  resuspend the cells with 1-2ml of complete media. 
(3)The cell suspension was added to T25 flask containing 6-8mL complete medium and put it into an incubator for erection culture.

Subculture:

① Centrifugation method: collect cells, centrifuge at 1000rpm for 5 minutes, discard the supernatant, add 1-2mL of culture medium and blow evenly, divide the cell suspension into a new T25 containing 8ml medium at a ratio of 1:2 bottle.

② Half-volume medium exchange method: After the culture flask is left standing for 5-10 minutes, half of the supernatant medium is gently aspirated, and the remaining medium with cell sediment is resuspended and mixed, and the cell suspension is divided into 1:2 ratio. In a new T25 flask containing 8 ml of medium.

③ Pay attention to the change of pH value of media and cell density, renew the media regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 2×106个/ml.

Notes:
(1)The cells are density dependent. Initiate the subcultivation in T25 flask when the cell density reaches 80%; A subcultivation ratio of 1:2 is recommended. 
(2)If the cells in sealed culture bottle, put it into the incubator for cultivation after handling, with the cap unscrewed.

Recovery record:  According to the recovery instructions, the results of the cell recovery are reported as follows:

Item quality standard Recovery record
viability: suspension growth rate ≥ 80.0% in 96 hours adherence is observed in 18 hours, cell adherence rate ≥ 80.0% in 88hours
cell morphology: suspension, lymphoblast-like Suspension, lymphoblast-like, round
attached:
conclusion: good viability, no abnormal cell morphology, qualified ;

 

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