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NALM6 clone G5 human acute lymphoblastic leukemia cell G5 clone
suspension, lymphocyte-like
No. 359376
Product format: 2ml frozen vial x 2, or centrifuge tube 15mL
Biosafety level: 1,handle in ultra-clean table or safety cabinet
Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours. if it is overdue, it will be deemed as good receiving goods. The frozen storage tube shall be stored in a refrigerator at -80 ℃ after receiving the goods. If it is not used for a long time, it shall be transferred to liquid nitrogen storage overnight. The centrifuge tube is 15mL. After receiving the goods, the centrifuge tube shall be placed in the incubator for rest for 4 hours, and then the cells shall be subjected to routine operation. During recovery, each tube shall not be retained once used up. After recovery, it will be passed on to one generation and can be used normally. Please strictly follow this instruction, otherwise no reissue service will be provided if cell inactivation is caused.
Growth conditions:37 ℃,5% co 2,90% RPMI-1640 + 10% FBS. RPMI-1640:1640 culture solution, containing glutamine.
Resuscitation steps :
(1) 1 new 100mm plate containing 12mL of the above culture solution;
(2) the frozen storage tube is taken out of liquid nitrogen or -80 ℃, bathed in water at 37 ℃ for 1~2min, and moved into the safety cabinet for resuscitation as soon as possible after complete dissolution;
(3) using a sterile straw to suck the dissolved liquid into the new plate and shake well clockwise;
(4) put in (37 ℃,5% CO2) in the incubator, change the liquid overnight and be full in 5-7 days.
Subculture: gently blow the cultured suspension cells evenly, distribute them to 2~3 fresh culture liquid dishes, gently shake well and put them into incubator for culture;
Cryopreservation: transfer the suspended cells to a centrifuge tube, centrifuge (110g,3min), resuspend the cells with 3mL of cryopreservation solution (50% basal medium + 40% FBS + 10% DMSO) after centrifugation, blow evenly, divide them into 3 cryopreservation tubes, freeze them at -80 ℃ with a program cooling box, and transfer them to liquid nitrogen for storage overnight.
Recovery record: According to the recovery instructions, the results of the cell recovery are reported as follows: